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dna microarray analysis procedures  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna microarray analysis procedures
    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on <t>DNA</t> <t>microarray</t> data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.
    Dna Microarray Analysis Procedures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+analysis+procedures/Deoxyribonucleic+acid/pmc12621471-417-8-29
    Average 99 stars, based on 1 article reviews
    dna microarray analysis procedures - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer"

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102445

    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.
    Figure Legend Snippet: Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Techniques Used: Expressing, Microarray

    Related Articles

    Microarray:

    Article Title: Peroxisome Proliferator-activated Receptor γ Coactivator-1 α Isoforms Selectively Regulate Multiple Splicing Events on Target Genes
    Article Snippet: Microarray Procedures—For global analysis of gene expression, we used the Affymetrix GeneChip Mouse Exon 1.0 ST array (46). .. Microarray Procedures—For global analysis of gene expression, we used the Affymetrix GeneChip Mouse Exon 1.0 ST array (46). ..

    Article Title: Pulmonary arteries of Williams syndrome patients exhibit altered serotonin metabolism genes and degenerated medial layer architecture.
    Article Snippet: BACKGROUND: Williams–Beuren syndrome (WS) is characterized by cardiovascular abnormalities associated with a multigene deletion on 7q11.23, in particular elastin (ELN).. Peripheral pulmonary artery stenosis (PPAS) frequently affects pediatric patients with WS.. Molecular investigation of WS pulmonary arterial (PA) tissue is limited by tissue scarcity.

    Article Title: Partial IGF-1 deficiency is sufficient to reduce heart contractibility, angiotensin II sensibility, and alter gene expression of structural and functional cardiac proteins
    Article Snippet: As previously reported [ , , ], heart mRNA was isolated from animals belonging to the three experimental groups in accordance with the protocol outlined in RNAqueousH-Micro Kit (Ambion, USA). .. Technical procedures for microarray analysis, including quality control of mRNA, labeling, hybridization and scanning of the arrays were performed according to standard operating procedures for Affymetrix protocols (GeneChipH Expression Analysis Manual, Affymetrix, USA). .. The mRNAs were profiled using Affymetrix HT MG-430.

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Article Title: MiR-7684-5p leads to surgery-induced cognitive decline in mice probably through the downregulation of SorLA
    Article Snippet: .. Microarray experimental procedure A DNA microarray analysis was conducted to screen the differentially expressed genes from the hippocampal tissues (Affymetrix _7G_ miRNA 4.0 Array was used to analyze miRNA profiles). ..

    Gene Expression:

    Article Title: Peroxisome Proliferator-activated Receptor γ Coactivator-1 α Isoforms Selectively Regulate Multiple Splicing Events on Target Genes
    Article Snippet: Microarray Procedures—For global analysis of gene expression, we used the Affymetrix GeneChip Mouse Exon 1.0 ST array (46). .. Microarray Procedures—For global analysis of gene expression, we used the Affymetrix GeneChip Mouse Exon 1.0 ST array (46). ..

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    other:

    Article Title: Transcriptional basis of the acclimation to high environmental temperature at the olfactory receptor organs of Drosophila melanogaster
    Article Snippet: We note that a gene was considered to be expressed in the antennae in each group when it appeared as “Present” in the statistical detection calls for the four replicates in the group provided by the Affymetrix GeneChip® microarray analysis software (see the RNA extraction and microarrays subsection in the Methods section).

    Article Title: Peroxisome Proliferator-activated Receptor γ Coactivator-1 α Isoforms Selectively Regulate Multiple Splicing Events on Target Genes
    Article Snippet: Microarray Procedures For global analysis of gene expression, we used the Affymetrix GeneChip Mouse Exon 1.0 ST array ( 46 ).

    Article Title: A Novel Dual Antibody Staining Assay to Measure Estrogen Receptor Transcriptional Activity
    Article Snippet: Fig. 1 Breast cancer cell lines (T47D, MCF7) were deprived of estradiol for 48 h and subsequently stimulated with 1 or 10 nM estradiol, respectively, for 16 h. RNA was isolated and ER pathway activity was measured using Affymetrix U133P2.0 microarray expression analysis (see Methods for the used method).

    Labeling:

    Article Title: Pulmonary arteries of Williams syndrome patients exhibit altered serotonin metabolism genes and degenerated medial layer architecture.
    Article Snippet: BACKGROUND: Williams–Beuren syndrome (WS) is characterized by cardiovascular abnormalities associated with a multigene deletion on 7q11.23, in particular elastin (ELN).. Peripheral pulmonary artery stenosis (PPAS) frequently affects pediatric patients with WS.. Molecular investigation of WS pulmonary arterial (PA) tissue is limited by tissue scarcity.

    Article Title: Partial IGF-1 deficiency is sufficient to reduce heart contractibility, angiotensin II sensibility, and alter gene expression of structural and functional cardiac proteins
    Article Snippet: As previously reported [ , , ], heart mRNA was isolated from animals belonging to the three experimental groups in accordance with the protocol outlined in RNAqueousH-Micro Kit (Ambion, USA). .. Technical procedures for microarray analysis, including quality control of mRNA, labeling, hybridization and scanning of the arrays were performed according to standard operating procedures for Affymetrix protocols (GeneChipH Expression Analysis Manual, Affymetrix, USA). .. The mRNAs were profiled using Affymetrix HT MG-430.

    Hybridization:

    Article Title: Pulmonary arteries of Williams syndrome patients exhibit altered serotonin metabolism genes and degenerated medial layer architecture.
    Article Snippet: BACKGROUND: Williams–Beuren syndrome (WS) is characterized by cardiovascular abnormalities associated with a multigene deletion on 7q11.23, in particular elastin (ELN).. Peripheral pulmonary artery stenosis (PPAS) frequently affects pediatric patients with WS.. Molecular investigation of WS pulmonary arterial (PA) tissue is limited by tissue scarcity.

    Article Title: Partial IGF-1 deficiency is sufficient to reduce heart contractibility, angiotensin II sensibility, and alter gene expression of structural and functional cardiac proteins
    Article Snippet: As previously reported [ , , ], heart mRNA was isolated from animals belonging to the three experimental groups in accordance with the protocol outlined in RNAqueousH-Micro Kit (Ambion, USA). .. Technical procedures for microarray analysis, including quality control of mRNA, labeling, hybridization and scanning of the arrays were performed according to standard operating procedures for Affymetrix protocols (GeneChipH Expression Analysis Manual, Affymetrix, USA). .. The mRNAs were profiled using Affymetrix HT MG-430.

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Control:

    Article Title: Partial IGF-1 deficiency is sufficient to reduce heart contractibility, angiotensin II sensibility, and alter gene expression of structural and functional cardiac proteins
    Article Snippet: As previously reported [ , , ], heart mRNA was isolated from animals belonging to the three experimental groups in accordance with the protocol outlined in RNAqueousH-Micro Kit (Ambion, USA). .. Technical procedures for microarray analysis, including quality control of mRNA, labeling, hybridization and scanning of the arrays were performed according to standard operating procedures for Affymetrix protocols (GeneChipH Expression Analysis Manual, Affymetrix, USA). .. The mRNAs were profiled using Affymetrix HT MG-430.

    Expressing:

    Article Title: Partial IGF-1 deficiency is sufficient to reduce heart contractibility, angiotensin II sensibility, and alter gene expression of structural and functional cardiac proteins
    Article Snippet: As previously reported [ , , ], heart mRNA was isolated from animals belonging to the three experimental groups in accordance with the protocol outlined in RNAqueousH-Micro Kit (Ambion, USA). .. Technical procedures for microarray analysis, including quality control of mRNA, labeling, hybridization and scanning of the arrays were performed according to standard operating procedures for Affymetrix protocols (GeneChipH Expression Analysis Manual, Affymetrix, USA). .. The mRNAs were profiled using Affymetrix HT MG-430.

    Generated:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    RNA Extraction:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Reverse Transcription:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Fluorescence:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..



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    Image Search Results


    Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Journal: Materials Today Bio

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer

    doi: 10.1016/j.mtbio.2025.102445

    Figure Lengend Snippet: Kaplan-Meier overall survival analyses of low and high CD4, CD8A, CD8B, ADGRE1, and IL-6 expression in the tumors of TNBC patients based on DNA microarray data. CD4 low: n = 436; CD4 high: n = 430; CD8A low: n = 434; CD8A high: n = 432; CD8B low: n = 457; CD8B high: n = 409; ADGRE1 low: n = 140; ADGRE1 high: n = 34; IL6 low: n = 469; IL6 high: n = 463.

    Article Snippet: The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ].

    Techniques: Expressing, Microarray